منابع مشابه
Statistical evaluation of colocalization patterns in immunogold labeling experiments.
The ultrastructural localization of various antigens in a cell using antibodies conjugated to gold particles is a powerful instrument in biological research. However, statistical or stereological tools for testing the observed patterns for significant clustering or colocalization are missing. The paper presents a method for the quantitative analysis of single or multiple immunogold labeling pat...
متن کاملTheoretical considerations of immunogold labeling of cellulose synthesizing terminal complexes
The sodium dodecyl sulfate (SDS)-solubilized freeze fracture replica labeling (SDS-FRL) technique has been successfully applied to a vascular plant, Vigna angularis [Kimura et a!. 1999, Plant Cell II: 2075 2085] and a bacterium, Acetobaeter xylinum [Kimura et al. 2001, J. Bacteriol. 183: 5668 -5674] for understanding cellulose biosynthesis. However, we do not know yet how many gold particles ca...
متن کاملImmunogold Staining of Ultrathin Thawed Cryosections for Transmission Electron Microscopy (TEM).
INTRODUCTIONA pre-embedding method of immunochemical staining is used if antigens are damaged by resin embedding, or if the best preservation of membranes is required. Applying immunogold reagents to sections of lightly fixed tissue, free of embedding medium, can be a very sensitive method of immunochemical staining. Cells or tissues are fixed as strongly as possible and then treated with a cry...
متن کاملTransfer Learning for the Recognition of Immunogold Particles in TEM Imaging
We present a Transfer Learning (TL) framework based on Stacked Denoising Autoencoder (SDA) for the recognition of immunogold particles. These particles are part of a high-resolution method for the selective localization of biological molecules at the subcellular level only visible through Transmission Electron Microscopy (TEM). Four new datasets were acquired encompassing several thousands of i...
متن کاملImmunogold staining: adaptation of a cell-labeling system for analysis of human leukocyte subsets.
We have assessed the Immunogold Cell-Labeling System (IGS) for potential use in the clinical laboratory. In this technique, cell suspensions incubated with monoclonal mouse antibodies are reacted with anti-mouse antibodies labeled with colloidal gold. Surface marker cells, bearing dark blue-black granules, are easily distinguished by light microscopy. The percentages of T cells, T cell subsets,...
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ژورنال
عنوان ژورنال: Journal of Histochemistry & Cytochemistry
سال: 2002
ISSN: 0022-1554,1551-5044
DOI: 10.1177/002215540205000612